Cross-linked enzyme aggregates (CLEA) in enzyme improvement – a review
نویسندگان
چکیده
منابع مشابه
Cross-linked Enzyme Aggregates of Catalase from Bovine Liver
Stabilization of multimeric enzymes is one of the major challenges in biocatalysis, because dissociation of subunits can inactivate the enzyme. Catalase (CAT; EC 1.11.16) is a homotetramer containing Fe-protoporphyrin IX in its active site. CAT breaks down hydrogen peroxide into water and molecular oxygen. In this study, crosslinked enzyme aggregates of bovine liver CAT (CAT-CLEAs) were prepare...
متن کاملUse of Cross-Linked Enzyme Aggregates (CLEAs) for performing biotransformations
The methodology for the preparation of cross-linked enzyme aggregates (CLEAs) and their application as highly effective, recyclable biocatalysts in a wide variety of transformations is reviewed. The method constitutes a combination of enzyme purification and immobilization into a single operation and allows for the use of relatively crude enzyme preparations. The resulting CLEAs have excellent ...
متن کاملCross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts.
The key to obtaining an optimum performance of an enzyme is often a question of devising an effective method for its immobilization. In the present review, we describe a novel, versatile and effective methodology for enzyme immobilization as CLEAs (cross-linked enzyme aggregates). The method is exquisitely simple (involving precipitation of the enzyme from aqueous buffer followed by cross-linki...
متن کاملHybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis
Novel hybrid magnetic cross-linked enzyme aggregates of phenylalanine ammonia lyase (HM-PAL-CLEAs) were developed by co-aggregation of enzyme aggregates with magnetite nanoparticles and subsequent crosslinking with glutaraldehyde. The HM-PAL-CLEAs can be easily separated from the reaction mixture by using an external magnetic field. Analysis by scanning electron microscopy (SEM) and confocal la...
متن کاملCross-Linked Enzyme Aggregates of Naringinase: Novel Biocatalysts for Naringin Hydrolysis
Cross-linked enzyme aggregates (CLEAs) have emerged as interesting biocatalyst design for immobilization. These new generation enzyme biocatalysts, CLEAs, in addition to exhibiting good mechanical stability, can be highly active, since they do not include large amounts of foreign particulate nonenzymatic material and may have increased stability. Naringinase (NGase) is an enzyme complex with hi...
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ژورنال
عنوان ژورنال: Biocatalysis
سال: 2016
ISSN: 2353-1746
DOI: 10.1515/boca-2015-0012